In September 1992 Darren Knight Gallery opened with a group exhibition at 65 Smith Street, Fitzroy, Melbourne. Exhibiting mainly Australian and New Zealand artists, the gallery operated from this small shop front space for four years before relocating to Sydney.
In February 1997 Darren Knight Gallery Sydney opened in a former pleating factory at 840 Elizabeth Street, Waterloo, the first gallery to establish itself in what is now a thriving arts precinct.
In 2003 renovations provided a second gallery space and stockroom on ground level.
Darren Knight Gallery is a regular participant at local art fairs and has exhibited at international fairs including, ARCO Madrid 2002, The Armory Show, New York 2006, NEXT Chicago 2008, Auckland Art Fair 2011 & 2013 and Art Basel Hong Kong 2015.